![]() ![]() ![]() Trial registration: Chinese Clinical Trial Registry, ChiCTR2000032211. Salvage CART2 provides an opportunity for bridging to transplantation and long-term survival. Mixed infusion of CD19- and CD22-targeted CAR-T cells is a safe and effective regimen for children with B-ALL who relapse after prior CD19-targeted CAR-T therapy. Cytokine release syndrome (CRS) only occurred with a grade of ≤ 2, and no patients experienced symptoms of neurologic toxicity during CART2. 3 (pt03), CAR-T cells were still detected in the peripheral blood (PB) at 347 days post-CART2. Three of the five patients bridged to consolidated allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CART2 and remained in MRD-negative CR at the cut-off time. ![]() The median follow-up time was 26.3 months. The 6- and 12-month overall survival (OS) rates were 100%. ResultsĪfter CART2, all five patients had minimal residual disease (MRD)-negative complete remission (CR). Throughout the trial, we evaluated the patients’ clinical responses, side effects, and the expansion and persistence of CAR-T cells. CD19- and CD22-CAR lentivirus-transfected T cells were cultured separately and mixed before infusion in an approximate ratio of 1:1. In this study, we recruited five patients who relapsed after CD19-targeted CAR-T. Therefore, there is a need to explore the safety and efficacy of co-administration of CD19- and CD22-targeted CAR-T as a salvage second CAR-T therapy (CART2) in B-ALL patients who relapse after their first CD19 CAR-T treatment (CART1). However, poor results are obtained when the same product is reused in patients who relapse after CAR-T. CD19-targeted chimeric antigen receptor T-cell (CAR-T) therapy has shown remarkable efficacy in treating relapsed or refractory pediatric B-lineage acute lymphoblastic leukemia (B-ALL). ![]()
0 Comments
Leave a Reply. |