![]() ![]() Boronĭescription: Boron is a powdery, black solid. Due to its lightweight metal properties, it is used structurally in the aerospace industry. ![]() When combined, the resultant metal, beryllium copper, is used for springs and a variety of electrical applications. Where It's Used: This element is most commonly used as an alloying agent for copper. Berylliumĭescription: Beryllium is a lightweight metal. It is also used in aluminum alloys to make cookware more durable and, most surprisingly, as a mood stabilizer in psychiatric medicines. Where It's Used: Lithium is known most commonly to be used in batteries. Lithiumĭescription: Under standard conditions, Lithium is the lightest metal and is soft and reactive. Due to its wide recreational usage, natural sources of Helium are at risk of being completely depleted in the next decade, sparking fears for the scientific community. It is also used as an inert shield for arc welding and to pressurize liquid fuel tanks in rockets. Where It's Used: Helium gas is commonly known to be lighter than air, which leads to its use in weather and party balloons. Hydrogen was used as a main fuel for the Space Shuttle program by NASA and is currently used heavily by the petroleum and manufacturing industries.ĭescription: Helium is an inert gas and the second-lightest element. The chemical is used heavily as both a gas and liquid fuel. Where It's Used: Hydrogen makes up about 90 percent of the universe's atoms. Hydrogenĭescription: Hydrogen is an explosive gas and also the lightest element. Use this guide to help you identify the group of each metal as you learn more about them. Each element header has its name, atomic number, atomic symbol, and color code to indicate the group it belongs to.Īs an example, Hydrogen is green, so it belongs to the group "other nonmetals." ![]()
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![]() ![]() The Walking Dead Season 9 airs Sundays at 9 p.m. Whatever happens, one thing’s for sure: The Whisperers are officially here, and we’re likely about to say goodbye to at least a couple more characters. Rather, their friends may be brought to them, either as an offer for a trade or via their heads on pikes. This may suggest they won’t be the ones to find Luke and Alden. Tara insists they return to Hilltop and make a plan before looking for their missing friends. Alpha’s arrival comes one episode following the official Whisperer reveal, in which the new group of enemies disguised as walkers were first unveiled in deadly fashion, taking Tom Payne’s Jesus. Since the horses have been cut open, they know it’s likely the work of Whisperers. The most difficult thing to get right about the Whisperer masks the eyes. Members of Hilltop, including new leader Tara and Magna and her group, find Luke and Alden’s horses. What if Luke and Alden are the main attractions of the fair? Luke even says they’ll “knock ‘em dead at this fair,” which could be foreshadowing their fates.ĪMC has released a sneak peek (above) for the next episode. The AMC TV series has put twists on storylines from the comics before, and it’s possible this could be another instance where different characters suffer a gruesome fate. In the comics, the fair is when the Whisperers put the heads of several characters, including Ezekiel and Rosita, on pikes to mark their territory. ![]() Luke decides that since he and Alden both have musical talents, they should perform at the upcoming fair. However, it’s more likely that their conversation from earlier in the episode could play a role in their fates. It’s possible they could simply be part of a trade for Lydia, Alpha’s daughter. Luke and Alden are now the Whisperers’ hostages. The cast and the creators take you behind the scenes and break down each horrifying battle of the survivors ongoing fight against the Whisperers.Watch from. The episode ends with Alden and Luke surrounded by The Whisperers and introduces their leader, Alpha, wearing a walker mask and holding a shotgun. ![]() Luke’s convinced the trail will lead them to their missing friends, but instead, it leads them right into a trap. Tony WE'RE COMING IN HOT THIS MONTH II III Dear TWD Crew, When I look back, I was so sure that Rick was going. Alden partners up with Luke, and the two come across Yumiko’s arrows. Carol cant fix the mess the writers of the show have now created, just. While Michonne and the others are gone, however, the people at Hilltop are worried about their friends and send out scouts. Catch up on the latest TWD episode available NOW on AMC+ plus unlimited music. If you've been used to one set of rules for zombies, and you haven't really encountered ones that do these other things, then some of your mechanisms for protecting yourself change.Alden and Luke follow the arrows into a trap in 'The Walking Dead' Season 9 Episode 9. ![]() "We're always looking for ways to kind of turn up the heat on our heroes. As showrunner Angela Kang pointed out during our exclusive chat: 130, are a group of survivors who have pushed through the zombie apocalypse by wearing suits made from human flesh, designed to. Admittedly, it's not the best explanation, but it does make more sense than anything else fans have come up with to try and explain those earlier "smart" zombies away.Īnd another benefit of this is how these new variants have reinvigorated the show during its final run. The Whisperers, first introduced in 2014’s The Walking Dead No. So these smarter variants have always existed within the show, but our gang has just been lucky enough to avoid them up until now. instagram 0 followers Top Reviews The Walking Dead Swear Review November 27. We're kind of playing into the idea that some of these are out there and they're just encountering them now." Luca Whispers Into Gemma Owens Ear, Love Island Fans Freak All News. "We sort of played into that as if there might be certain walkers in certain regions that might have different abilities that we really haven't seen before. ![]() ![]() ![]() No-frills product, easy to use and friendly to newbies.The range of input formats is broad, from CD, DVD to HD DVD and Blu-ray disc.Rip DVD to Video_TS folder with fast speed and low CPU consumption."AnyDVD is unable to crack all CSS Keys on this disc!" message points out the striving direction for AnyDVD HD. 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With the release of v1.6.7, it added support for new DVD copy protections. With the release of v1.5.4, it updated Blu-ray keys for AACS v17. With the release of v1.01 build 646, it improved recognition of bogus titles on DVD discs with structure protection. ![]() With the release of v1.01 build 634, it improved handling for DVD discs with structure protection. MakeMKV has been working on improving its DVD and Blu-ray decryption capabilities. Other DVD Decrypters You can Use to Unencrypt DVDs 1. How to Decrypt Copy-Protected DVDs with WinX DVD Ripper Platinumĭisclaimer: Digiarty Software does not advocate ripping copy-protected DVDs for any commercial purpose and suggests referring to the DVD copyright laws in your countries before doing so. Specify destination folder and click "RUN" to begin decrypting and copying protected DVD collection to MP4. You can skip this and move to step 4 directly. This is an optional step primarily suited to advanced users. 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Offer complete yet advanced GPU hardware acceleration to push DVD ripping speed up to 50% faster than before.Copy DVD to ISO image, backup DVD to MPEG-2 file and clone DVD with main/full title at 1:1 ratio.Decrypt DVD collection to MP4, H264, MKV, MOV, AVI, iPhone, iPad, Android, Xbox, PS4, etc.Remove DVD protection from disc, including CSS, AACS 2.0, Region Code 1-6, UOPs, RCE, Disney X-project DRM, Sony ARccOS, etc. ![]() ![]() ![]() The point is that I had high expectations along with the two friends for whom I purchased great seats in the center/right Orchestra sections and we were all severely disappointed by the program. Personally, I love all of it but, still found this an odd decision. I would again imagine that this was the musical director’s choice but, it left many in the audience not connecting to the more obscure material. As amazing as Aida Cuevas is, was there a reason that she chose not to cover Ronstadt’s most popular Spanish language songs, La Cigarra and La Charreada. The final issue revolves around song selection. With all of the fine musicians and vocalists onstage, it would be fascinating to know whose decision it was to eliminate those much needed backing vocals. When Will I be Loved stood out most in that regard and felt empty even though La Marisoul gave it her all as usual. The final element missing was the necessary backing vocals (harmony, doubling, etc) required to truly flesh out some of the great rock songs. It appeared from the MC that she might have been a last minute addition which indicates that someone else was slated to sing her part and canceled. Perhaps she was frightened on stage or simply not connected to the style she was being forced to sing. ![]() Souza's work with Jazz ensembles and she has a great instinct. Anyone paying tribute to Linda Ronstadt requires intense musical chops and she was not up to the task in any way. The second is that one of the primary vocalists (Luciana Souza) was woefully unprepared and of shockingly weak voice (pitch issues, lack of confidence, etc). The first was that the show seemed quite brief in its two segments given the breadth of material from which the musical director had to choose. There were four overwhelming problems and I was not the only one to experience them. As a musician, I was impressed with the quality of the players who were as good as one could imagine. I have been to the Soraya several times over the past few years (back when it was named the VAPC) and was so excited to attend my first indoor concert since pandemic lockdown. (Kansas City Star)Ī powerful voice that encapsulates the essence of her nation’s spirit”.The Soraya (The Valley's Center for the Performing Arts) - Northridge “An esteemed figure, she is to Mexico what Aretha Franklin is to the United States:Ī powerful voice that encapsulates the essence of her nation’s spirit”. 2, (2020 & 2021) feature her interpretations of the timeless songs made famous by the iconic 1930s ranchera singer Lucha Reyes and both albums made a GRAMMY® nomination again for “Best Mariachi/Ranchero Album” category. Her two latest albums, Antología de la Musica Ranchera Vol. An artist known widely as the “Queen of Ranchera Music,” and “La Voz de México” (the Voice of Mexico) in her native country, she celebrates her 47th year as performer with this special tour, "Yo Creo Que Es Tiempo” (“I Think It’s Time”), named for her 1983 hit written by her late mentor and producer, pop icon Juan Gabriel. Over her career, she’s recorded an impressive 41 full-length albums that have sold internationally in excess of 11 million copies.Īn international icon of Mexican music since she was a teenager, Aída Cuevas is an impassioned performer with a striking voice that evokes life’s deepest sorrows, greatest joys and most profound longings. Much like Ella Fitzgerald’s classic releases for Verve, her “songbook” albums have played a central role in defining Mexican popular music. Cuevas is the first and only one female singer in the traditional mariachi genre to ever win a GRAMMY® and a Latin GRAMMY Award® and now celebrates a long lasting career which includes eleven GRAMMY® nominations in the “Best Mariachi/Ranchero Album” category. Aída Cuevas, known as "The Queen of Mariachi Music", celebrates 47 years singing traditional Mexican and Latin American music. ![]() ![]() ![]() If you see a request to "Delete permanently" when you try to move an item to trash, your trash is full. To delete it, use your device’s gallery app. If you see a photo or video in Google Photos that you think you deleted, it might be on a removable memory card.If you delete an item from your Android 11 and up device without it being backed up, it will stay in your trash for 30 days. ![]() If you delete a photo or video that’s backed up in Google Photos, it will stay in your trash for 60 days.Learn what happens to photos you've deleted Tap and hold a photo or video you want to move to the trash.On your Android phone or tablet, open the Google Photos app.Learn how to remove backed up photos and videos from Google Photos but not your device. Important: If you back up photos and videos to Google Photos on a mobile device, when you delete them from the Google Photos app, they’re also deleted from your device. We should all be able to recover data from our Mac hard drives if something goes wrong.Download and install the Google Photos app. Instead, quit using it and download and install data recovery software on another disc or partition. If you keep using your drive or partition, your files will be overwritten and you won’t be able to recover them. The key is to remain calm and to immediately cease using the disc where you will be attempting data recovery. Whatever the reason, if you lose or erase data on your Mac, you’ll need to utilize file recovery software. Human mistake is always a possibility, such as forgetting you needed a file just after you emptied the trash or recycling bin. Upgrades to the operating system or software applications might result in files being lost or destroyed accidentally. Physical or logical damage to your computer’s hard disk might cause files to vanish. The features of the free edition are limitedĮven if you follow best practices and frequently back up your laptop, you may need to recover lost or missing information for a variety of reasons. The user interface resembles that of a file manager The free version has limited functionalityĭM Disk Editor and Data Recovery Software Here is the list of limitations of the all-listed software along with their prices to help you choose the relevant product: S.No Only file previews are available for free.Stellar Photo Recovery will most likely be able to find the file you’ve lost on a broad range of devices because of the extensive list of supported file types and cameras/storage devices. It can also recover data from the newest DSLRs, Mirrorless Cameras, and video cameras, as well as 4k, 6k, 8k, UHD, and/or 360-degree files, CFast, CompactFlash, microSDHC/XC cards, and CFast, CompactFlash, microSDHC/XC cards (when a memory card is inserted and you connect it to a computer via USB). The Stellar Photo Recovery program restores images, videos, and music data deleted, corrupted, formatted, or unavailable due to a virus or inaccessible discs. We attempted to assess the tools that garnered the most positive feedback and left users happy with their overall experience. User reviews – If you’re looking for information about software solutions, the user community is a wonderful place to start. Popularity – We wanted to check if the most popular recovery solutions’ popularity was justified by their functioning and feature set. ![]() ![]() ![]() Despite her success, she seems unhappy at times, desperately craving the attention of her busy parents. She is very sensitive about how her first name is pronounced and she is the most popular girl at school. At first, an arch-nemesis to Molly, she eventually becomes her friend.
![]() ![]() The season ended with a controversial finish, with the two title rivals for the drivers' crown entering the last race of the season with equal points. Mercedes retained the Constructors' Championship for the eighth consecutive season. Four-time defending and seven-time champion Lewis Hamilton of Mercedes finished runner up. Honda became the second engine supplier in the turbo-hybrid era to power a championship winning car, after Mercedes. Verstappen became the first ever driver from the Netherlands, the first Honda-powered driver since Ayrton Senna in 1991, the first Red Bull driver since Sebastian Vettel in 2013 and the first non-Mercedes driver in the turbo-hybrid era to win the World Championship. By 2020 the schedule had grown to 22 world championship grands prix.Mercedes won their eighth consecutive Constructors' Championship.Īt season's end in Abu Dhabi, Max Verstappen of Red Bull Racing- Honda won the Drivers' Championship for the first time in his career. The first F1 season took place in 1950, when seven races were held. The calendar has more than tripled in length since the world championship began. Get daily updates from RaceFans by email.Stay up-to-date with which races are confirmed on the 2021 F1 calendar by following RaceFans on social media: ![]() Get the latest on next year’s F1 calendar The Vietnam Grand Prix, which was supposed to take place for the first time in 2020 but was cancelled due to the pandemic, will not go ahead in 2021. It intended to hold the race in May, but no further details have been confirmed. In October 2019 F1 announced ‘agreement in principle’ to hold a race on a new circuit at the Hard Rock Stadium in Miami. The first Saudi Arabian Grand Prix will take place on December 5th. What new races will be on the calendar? Miami had hoped to join the 2021 F1 calendarįormula 1 has confirmed a new night race will take place on the streets of Jeddah. Import the race dates to your preferred calendar via this link: This will be updated when further details are issued. The dates for the 2021 races, tests and car launches announced so far have been added to the RaceFans F1 calendar. A single three-day test will be held at Bahrain International Circuit from March 12th to 14th. Pre-season testing has been reduced from six days to three for the 2021 championship. However other new races the sport aspired to hold in Miami, USA and Hanoi, Vietnam did not join the schedule as hoped.Īdvert | Become a RaceFans supporter and go ad-free The Dutch Grand Prix, which was supposed to return at Zandvoort in 2020 but fell foul of the pandemic, was confirmed for a September date. ![]() The 73rd running of the world championship will also see the addition of a new race in Saudi Arabia on the streets of Jeddah. However no replacement was arranged for Japan’s round, meaning the final calendar was shortened to 22 races. The cancelled race in Australia was eventually replaced by F1’s first race in Qatar. The original calendar also included a race at an undetermined venue which, three weeks before the season was due to begin, was confirmed as the Autodromo do Algarve in Portugal. However the later cancellation of Singapore’s round of the world championship opened up a new space on the calendar which was taken by Turkey.Īfter the season began in Bahrain the championship raced at Imola in Italy, replacing China. An extra race in Austria was added instead. The Canadian round was originally replaced by a new race in Turkey, but within 16 days F1’s trip to Istanbul had also been called off as the rising Covid-19 infection rate led the British government to impose severe travel restrictions. These also included China, whose race was called off early in the season, Canada, Singapore and Japan – the latter coming despite the Tokyo Olympics going ahead while the country was in a statement of emergency. The Australian round was later cancelled, joining a host of other races beyond F1’s European heartland which were scratched from the schedule. The season began there on March 28th, following three days of testing at the same Bahrain International Circuit. The Australian Grand Prix was delayed until November, and Bahrain became the new opening race. The first of those had to be postponed, however. While the 2020 season had been heavily delayed and curtailed, from the outset F1 stated its intention of running a more normal schedule in 2021, albeit with a record-breaking 23 races. ![]() ![]() We’ve also included the STABILITRAK Electronic Stability Control System with rollover mitigation to help your vehicle respond to your steering commands and TRACTION CONTROL. In fact we’ve provided you with an available REARVIEW CAMERA SYSTEM on select models that’s designed to help keep certain stationary objects like bikes in view when backing up. We considered your safety from every angle. While operating the 2012 chevy Equinox in any capacity it stalls. Plus enjoy the many features that Onstar has to offer. The 2012 Chevrolet Equinox has 328 NHTSA complaints for the engine at 63,969 miles. Stay organized with numerous storage locations like the Equinox’s deep CENTER CONSOLE DASHBOARD STORAGE COMPARTMENT and rear seat map pockets. The 2012 Chevy Equinox SUV has a Manufacturers Suggested Retail Price (MSRP) starting just over 24,000 for the base. But some reviewers dislike that the Equinox lacks an optional. 2012 Chevrolet Equinox Read Owner And Expert Reviews, Prices, Specs www. Available POWER-PROGRAMMABLE LIFTGATE adjusts to your height and can be conveniently opened by remote. The 2012 Chevrolet Equinox earns great feedback because of its useful organizational and cargo features and spacious second row. 8 available versions of Chevrolet Equinox 2016. Check prices & reviews on aftermarket & stock parts for your 2012 Equinox Battery. The reclining 60/40 split-folding rear seatback gives you flexibility when storing cargo. Compare 2012 Chevrolet Equinox Battery brands. Rear passengers can relax in a standard MULTIFLEX SLIDING REAR SEAT which travels nearly eight inches. ![]() ![]() The Chevy Equinox offers more rear seat leg room than any competitor. The 2012 Chevy Equinox is The Crossover that fits your life! It’s fuel-conscious with front-wheel-drive models offering an EPA estimated 32 miles per gallon highway with the standard 2.4 liter ECOTEC four-cylinder engine. ![]() ![]() ![]() His Inbox came over fine, but all his other folders came over empty. Note: the arguments are passed to : -M "%~1" -M "%~2" -M "%~3" -M "%~4" -M ". I had an IMAP user who migrated to Surgemail a while back. Mailsend -q -f -smtp xyz.com -user -pass xxxxx -name "Steffen L" -t -sub "Lets Encrypt mod_md notification" -M "%~1" -M "%~2" -M "%~3" -M "%~4" -M "." Xcopy \md\domains\privkey.pem \ssl\surge_priv.pem /Y Get-AppxPackage -Name microsoft.windowscommunicationsapps Remove-AppxPackage. Read reviews and product information about Microsoft Outlook. Xcopy \md\domains\pubcert.pem \ssl\surge_cert.pem /Y Im not sure if it will work though if it is not run as the user in question. Find the top-ranking alternatives to NetWin SurgeMail based on 46450 verified user reviews. REM copy certificates to mail server (in my case Surgemail) (SSL Secure version) - Web interface for domain administrators for creating new accounts, modifying passwords, forwarding rules etc. ![]() REM kill eventually zombie php-cgi.exe's when you run php with mod_fcgid Setting, log in to the user self management interface: Password, edit any of the user's information including You have looked up an existing user you can change a user's Page that allows you to lookup, create, modify and search for Managing Accounts via the Web or API/httpĬreate or sign-up for accounts via the web interface on the userĭifferent methods, the method being used is specified per domainĭone via the "User accounts" option in the web admin contents.REM Remove following if you do not want to stop/start Apache Sql, etc.) then the backend database can be used directly.Įxamples to experiment with save them as htm files and open them For example here is a simple HTTP formĬonsider the other two methods available to interface withĪccounts, which method you choose will depend on your preciseĬan be talked to directly using the authent protocol Ģ) if the authent module is using a back end database (ldap, Options elsewhere you can do so by interfacing with it using If you want to provide some or all of these System is an HTTP based system using GET and POST commands to To remove accounts you will need to edit and manually remove You can create additional system admin accounts for surgemail This users page for all the domains they manage. surgewalllocaltoo - For web domain admin try local database too surgewalloptions - SurgeWall miscellaneous options (Version 1. To use from a program you need to send the formĪs an http POST using tcpip. ![]() Mechanism for easily sending web 'forms' from a program so will For these functions you must have the web admin This group of forms provide simple access to the authent moduleįunctions directly, this can be used to add/del/modify/search userĪccounts. Added tellmail mirroranalyze, which counts domains,users,messages on both hosts, the figures are displayed as lcl,rmt in tellmail mirrorstatus output. david camm advanced web systems keller, tx ps. when you click on any one of them the next screen has a delete button. just click on domain settings and you'll get a list of your domains. do NOT click the next to domain settings. Otherwise, the client is sent this value. Subject: Re: SurgeMail List Removing a domain. If the Domain Name is left blank, then the domain name of the firewall it sent to the client. Do not send the new Step 2 Remove suspicious email forwarding addresses. 7.7b4 Fixed serious login delay if GSAFEWARNING setting enabled in some situations. The dhcp docs say the following about the domain name field in the DHCP settings screen: Domain Name Specifies the domain name passed to the client to form its fully qualified hostname. Check if a domain name is classified as potentially malicious or phishing by. (since that can indicate this server doesn't have real data). User/password and send it using the normal http basicĪuthentication method. SurgeMail Change History 7.7c New Beta 7.7b5 Added code to automatically disable resyncprune and repair feature if downtime exceeds 24 hours. ![]() ![]() Cells were incubated for 15 min at room temperature in the dark and then centrifuged as described above. Secondary antibodies were diluted in PBS containing 0.02% NaN 3 and 1% inactivated FBS. Supernatant was decanted and cells were labeled with 100 μL of a cocktail containing 4 secondary isotype-specific antibodies conjugated to fluorochromes (listed in Table 1). Cells were incubated for 15 min at room temperature and centrifuged (400 × g at room temperature for 2 min). Monoclonal primary antibody diluted in PBS containing 0.02% NaN 3 and 1% inactivated FBS was added to individual wells in 50 μL aliquots. Primary and secondary antibodies used for flow cytometric-staining are listed in Table 1. Expression of Activation Molecules on B Cells Isolated from Lymph Nodes The change in optical density readings was calculated by subtracting the mean optical density readings for wells receiving coating buffer or PBS alone from the mean optical density readings for antigen-coated wells receiving the same test sample. The reaction was stopped by addition of sulfuric acid (0.18 M) and absorbances (450 nm) of individual wells measured (ELISA plate reader, Molecular Devices, Menlo Park, CA). Wells were then washed with PBST and incubated for 4.5 min at room temperature with substrate (3,3′5,5′-tetramethylbenzidine Kirkegaard and Perry Laboratories). After incubating for 20 h at 4☌ with test samples, wells were washed with PBST and incubated for 1 h at 37☌ with horseradish peroxidase-conjugated antibovine IgG heavy and light chains (Kirkegaard and Perry Laboratories) in PBS plus 0.1% gelatin. Optimal dilutions of test sera were determined by evaluation of the reactivity of 2-fold serial dilutions ranging from 1:6 to 1:800. Test and control sera were diluted in PBS containing 0.1% gelatin. After incubation for 1 h at 37☌ in the blocking solution, wells were washed in PBST and test sera were added to wells (100 μL/well). Plates were washed 3× with PBS with 0.05% Tween 20 ( PBST 200 μL/well) and blocked with a commercial milk diluent/blocking solution (200 μL/well Kirkegaard and Perry Laboratories, Gaithersburg, MD). Plates, including control wells containing coating buffer or PBS alone, were incubated for 15 h at 4☌. Microtiter plates (96-well Immulon II, Dynatech) were coated with OVA or WCS-PK (100 μL/well). The concentration of WCS-PK used in the ELISA was 40 μg/mL diluted in carbonate/bicarbonate coating buffer (pH 9.6). ![]() The concentration of OVA used in the ELISA was 1.56 μg/mL diluted in PBS. The relative amounts of Ig to OVA and WCS-PK in serum and in culture supernatants were determined by a capture ELISA. Mycobacterium bovis strain bacillus Calmette-Guerin.Additional studies are necessary to determine whether maternal immunologic experience transferred via colostral immunoglobulin inhibits production of mycobacteria-specific immunoglobulin production in the calf. In conclusion, calves generated B cell responses to ovalbumin and BCG after vaccination. Interestingly, IgM + cells isolated from the superficial cervical lymph node draining the vaccination site, but not from the opposing cervical lymph node, responded to antigen stimulation in vitro. An increased expression of major histocompatibility class II on CD5 +IgM + cells after stimulation was the only exception. Expression of activation molecules on ovalbumin- and purified protein derivative-stimulated CD5 +IgM + cells was similar to expression on the larger IgM + cell population. Stimulation of the same population with purified-protein derivative increased CD25 and CD80 expression on IgM + cells. Lymph node cell populations stimulated with ovalbumin had decreased CD5, CD21, and CD40 expression and increased B-B2, CD25, and CD80 expression on IgM + cells. In the second experiment, 6 calves were vaccinated with ovalbumin at 3 and 5 wk of age in addition to BCG at 3 wk of age. Vaccination with BCG did not elicit a measurable antibody response. ![]() Vaccination of 3-wk-old calves with ovalbumin elicited antigen-specific IgG 1 and IgG 2 anti-body responses that were amplified by secondary vaccination. Ovalbumin-specific IgG 1 and IgG 2 were not detected before vaccination. Mycobacterium bovis lipoarabinomannan-reactive IgG 1 and IgG 2 were detected in calf sera prior to vaccination, indicative of colostral transfer of maternal Ig cross-specific to BCG. ![]() Three of the 6 calves also were vaccinated with Mycobacterium bovis, strain bacillus Calmette-Guerin (BCG) at 3 wk of age. In the first of 2 experiments, 6 calves were vaccinated with ovalbumin at 3 and 5 wk of age. The objective of this research was to evaluate the effects of early vaccination on the phenotype (i.e., activation marker expression) and functional capacity of B cell populations in neonatal calves. ![]() |